Shared Facilities

Angio BioCore

Services

The Angio BioCore (ABC) is a state-of-the-art facility that has been established to conduct validated and highly reproducible in vitro and in vivo angiogenesis, hematopoietic and multi-parametric flow cytometry assays. These assays function as experimental platforms for understanding the basic mechanisms of hematopoiesis, angiogenesis and discovering compounds that inhibit new blood vessel formation in tumor microenvironments.

The specific duties of the ABC are:

  • Provide consultation and guidance for the design and analysis of experiments to define cell subsets that participate in angiogenesis (biomarkers for a variety of human diseases)
  • Prepare research and human clinical blood samples for acquisition in the Flow Cytometry Resource Facility and conduct detailed cell surface analysis using FlowJo software in ABC
  • Perform in vitro assays for angiogenesis
  • Perform in vitro clonal assays of hematopoietic progenitor cell function
  • Instruct interested investigators in the methods of hematopoietic stem and progenitor cell analysis and in a variety of angiogenesis assays
  • Develop new methods for detecting pro-angiogenic cells (non-vessel forming) and angiogenic cells (vessel-forming) from patient blood and tissue samples

Chargeback rates for services of the Angio BioCore, effective July 1, 2014, are below:

Cell Separation

Internal IU Faculty rates

External rates Services
$45 TBD Ficoll Preparation of Human Blood MNCs (20mls)
  • Preparation includes underlaying of up to 20mls of blood, washes and cell count
$105 TBD Ficoll Preparation of Human Blood MNCs (100mls)
  • Preparation includes underlaying of up to 100mls of blood, washes and cell count
$75 TBD CPT Preparation of Human Blood MNCs
  • Preparation includes processing of up to 48mls of blood in CPT tubes (supplied by the user), washes and cell count
$11 TBD Plasma Separation and Freeze Down
  • Preparation includes centrifuging and freeze down of up to eight vials of plasma
$11 TBD MNC Separation and Freeze Down (with Flow Cytometry)
  • Preparation includes freeze down of up to five vials of MNCs
$65 TBD MNC Separation and Freeze Down (without Flow Cytometry)
  • Preparation includes isolation of MNCs and freeze down of up to five vials of MNCs
$495 TBD Isolation of CD34+ Cells Via MACS Sort
  • Ficoli Separation of Cord Blood, Staining of MNCs with indirect CD34+ MACS kit
  • Cyopreservation of isolated cells per 1e6
  • Analysis of the cells for purity using FlowJo 1e6 cells
Cell Culture
Internal IU Faculty rates External rates Services
$35 TBD Quantitation of CFU-GEMMs from Human Blood
  • Plating, culture and quantitation of CFU-GEMMs (in triplicate)
  • Isolation of MNCs is a separate charge
$255 TBD Quantitation of Endothelial Outgrowth Cells (EOCs) from Human Blood
  • Plating, maintanence and quantitation of three wells for EOCs
  • Isolation of MNCs is a separate charge
$200 TBD Expansion and Cryopreservation of Endothelial Outgrowth Cells (EOCs)
  • Expansion of EOC colonies to passage 2 and cryopreservation of cells
Multi-Parametric Flow Cytometry
Internal IU Faculty rates External rates Services
$180 TBD 7-Color Analysis of Human Blood MNCs
  • Staining of MNCs with seven antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo
  • Does not include cost of data collection in Flow Cytometry Core
  • Isolation of MNCs is a separate charge
$122 TBD Brief Endothelial Phenotyping of Endothelial Cells
  • Staining of cell line with endothelial antigen panel to confirm phenotype, including controls
  • Does not include usage charge from Flow Cytometry Core
TBD TBD Up to 8-Color Analysis of Human Blood MNCs, Bone Marrow MNCs or Cell Lines

Can design any panel for the specific PI's interests

  • Staining of MNCs with eight antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
  • Does not include usage charge from Flow Cytometry Facility
  • Isolation of cells is a separate charge
Functional Assays
Internal IU Faculty rates External rates Services
$395 TBD Endothelial Cell Proliferation Assays (96 well format)
  • Thymidine incorporation in 96 well plates for up to 10 conditions (including controls) with 6 replicates
  • Does not include cost of cell line
$530 TBD Endothelial Cell Migration Assays
  • Haptotaxis in transwells for up to eight conditions (including controls) in triplicate
  • Does not include cost of cell line
$345 TBD Tube Forming Assays (Matrigel) with Human Endothelial Cells
  • Up to 10 conditions in triplicate assayed for endothelial cell tube formation
  • Does not include cost of cell line
$720 TBD Assessment of Endothelial Cell Apoptosis via TUNEL
  • TUNEL assessment of endothelial cell apoptosis
  • Up to six conditions in triplicate, including controls
  • Does not include cost of cell line.
Cryopreserved Cell Lines
Internal IU Faculty rates External rates Services
$225 TBD Human Umbilical Cord Blood EOCs
  • 1e6 cells
$225 $340 Human Peripheral Blood EOCs
  • 1e6 cells
$150 NA HUVECs
  • 1e6 cells
$300 NA HMVECs
  • 1e6 cells
$350 NA PMVECs
  • 1e6 cells
Miscellaneous
Internal IU Faculty rates External rates Services
$30/hour   Technician time
$60/hour   Retrospective analysis of FCS3.0 files
  • Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell
    sub-populations of interest