The Bio-Plex 200 System with High Throughput Fluidics (HTF) Multiplex Array System is a microplate-based bioassay system that can perform multiplex analysis of up to 100 different analytes in a single sample of 25uL. The Bio-Plex is faster, more efficient, and uses less sample volume than currently available technologies such as ELISA and Western Blot.
The Bio-Plex technology uses microscopic beads dyed with different ratios of two fluorochromes that emit a color spectrum unique to that fluorochrome ratio. Each bead is also tagged with an antibody specific to a certain target. Up to 100 differentially dyed beads can be used in a single multiplex assay, each conjugated to a different antibody. These bead-antibody conjugates are incubated with the target sample, washed, and then incubated with biotin-labeled detection antibodies (BIOT/Ab) specific for secondary epitopes on the target. Samples are washed and then incubated with fluorescently labeled streptavidin reporter antibodies (SA/Ab), washed, and resuspended for analysis. The fluorescence of the bead-antibody-target-BIOT/Ab-SA/Ab complex is then read on the Bio-Plex by a red laser to identify the specific bead by its fluorescent signature and a green laser to identify beads that have bound to a target. The level of target bound is indicated by the intensity of the reporter signal (SA/Ab).
The Bio-Plex Core Facility is located in Dr. Orschell’s laboratory, R3-C335 (Walther Hall), 980 W. Walnut St.