$105

Ficoll preparation of human blood MNCs

• Preparation includes underlaying of up to 100mls of blood, washes and cell count

$65

CPT preparation of human blood MNCs

• Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count

$235

7-color poly-chromatic flow cytometry analysis of human blood MNCs

• Staining of MNCs with 7 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
• Does not include cost of data collection in Flow Cytometry Core
• Isolation of MNCs is a separate charge

5-color poly-chromatic flow cytometry analysis of human blood MNCs                                                                          

• Staining of MNCs with 5 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.                                                                                                                                                                   
• Does not include cost of data collection in Flow Cytometry Core                                   
• Isolation of MNCs is a separate charge

$125

Quantitation of CFU-ECs from human blood

• Plating, culture and quantitation of CFU-ECs (in triplicate)
• Isolation of MNCs is a separate charge

$235

Quantitation of ECFCs from human blood

• Plating, maintanence, and quantitation of 3 wells for ECFCs
• Isolation of MNCs is a separate charge

$140

Expansion and cryopreservation of ECFCs

• Expansion of ECFC colonies to passage 2 and cryopreservation of cells

$500

Endothelial cell isolation from human umbilical veins

• Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by poly-chromatic flow cytometry

$135

Brief endothelial phenotyping of endothelial cells by poly-chromatic flow cytometry analysis

• Staining of cell line with endothelial antigen panel to confirm phenotype including controls
• Does not include usage charge from Flow Cytometry Core

$270

Endothelial progenitor cell clonogenic assays

• Limiting dilution analysis of up to 6 conditions in triplicate

$320

Endothelial progenitor cell clonogenic assays

• Single cell analysis of up to 6 X 96 well plates
• Does not include sorting charge from Flow Cytometry Core
• Does not include cost of cell line

$210

Endothelial cell proliferation assays (96 well format)

• Thymidine incorporation in 96 well plates for up to 10 conditions (including controls) with 6 replicates
• Does not include cost of cell line

$500

Endothelial cell migration assays

• Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate
• Does not include cost of cell line

$245

Tube forming assays with human endothelial cells

• Up to 12 wells assayed for endothelial cell tube formation

$365

Co-culture tube forming assays using transwell system

• Up to 12 wells assayed for endothelial cell tube formation
• Does not include cost of ECFC line

$565

Ex vivo aortic outgrowth model of capillary structures

• Aortic ring outgrowth using up to 6 mice (up to 5 conditions including controls)
• Does not include the cost of mice used

$265

Retroviral transduction of primary human endothelial cells with fluorescently labeled proteins

• Retroviral transduction with eGFP or dsRed and expansion of cell line

$265

Cryopreserved human umbilical cord blood and peripheral blood derived ECFC cell line

$265

Cryopreserved HUVEC cell line

$440

Mouse matrigel plug model

• Placement of 1-2 matrigel plugs in up to 6 mice, hemoglobin content analysis of plugs
• Does not include cost of mice or per diems, which will be calculated and charged on an individual basis

$665

Transplantation of human endothelial colony forming cells into immunodeficient mice

• Placement of collagen/FN/ECFC implants into up to 4 NOD/SCID or NS2 mice
• Does not include histological analysis of implants
• Does not include animal or per diem costs

Assessment of endothelial cell apoptosis via TUNEL                                                        

• TUNEL assessment of endothelial cell apoptosis.                                                              
• Up to 6 conditions in triplicate, including controls                                                                
• Does not include cost of cell line.

Cost to be determined on an individual basis

Bone marrow contribution to tumor microenvironments utilizing chimeric mice with green fluorescent protein-labeled bone marrow cells