Angiogenesis, Endothelial & Pro-Angiogenic Cell CorePolicies & Procedures

Acknowledgment, authorship and collaboration

All research work performed by the AEPCC should be acknowledged in all ensuing publications. Fees paid for services provided by the AEPCC should not negate the potential of co-authorship by AEPCC scientists. These acknowledgements and achievements are important for the existence and continued funding of the AEPCC. Given that many validation procedures and AEPCC offered services require advanced intellectual involvement by the AEPCC's scientists (examples below), it is expected that this acknowledgement will be in the form of co-authorship according to the guidelines for authorship recommended by the International Committee of Medical Journal Editors.

AEPCC scientists performing one or more of the following tasks meet the criteria for co-authorship:

  • Literature reviews and providing assistance in conceiving/selecting the experiments appropriate for the study goals.
  • Experimental/protocol design.
  • Assay optimization/development
  • Data analysis and interpretation of data (or both).
  • Preparation of figures or pertinent sections for the manuscript and revisions to address reviewer questions/concerns.

All contributions by AEPCC scientists that do not meet the criteria for authorship should be listed in the acknowledgments section. Examples of the tasks requiring an appropriate mention in the acknowledgment section include:

  • Mere collection of data (i.e. flow cytometry, Matrigel, TUNNEL)
  • Using PI-provided protocols that require no further optimization and/or trouble-shooting.
  • Mere collection of study data in which no data analysis or interpretation by AEPCC scientists is required.
  • Routine sample processing (i.e. isolation of cord blood, peripheral blood or bone marrow mononuclear cells, serum/plasma separation and storage etc).

Consultation Services

The Angiogenesis, Endothelial & Pro-Angiogenic Cell Core provides consultation services for IU Simon Cancer Center members interested in conducting validated and highly reproducible in vitro and in vivo angiogenesis assays. 

To initiate the consultation process, please complete a consultation form and e-mail it to Jamie Case, PhD, core director. Then contact the core to further discuss your project.

Chargeback rates for services of the Angiogenesis, Endothelial & Pro-Angiogenic Cell Core, effective July 1, 2011, are below:

Charge* Services

$100

Ficoll preparation of human blood MNCs

  • Preparation includes underlaying of up to 100mls of blood, washes and cell count

$65

CPT preparation of human blood MNCs

  • Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count

$220

7-color poly-chromatic flow cytometry analysis of human blood MNCs

  • Staining of MNCs with 7 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
  • Does not include cost of data collection in Flow Cytometry Core
  • Isolation of MNCs is a separate charge
$180

5-color poly-chromatic flow cytometry analysis of human blood MNCs                                                                          

  • Staining of MNCs with 5 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.                                                                                                                                                                   
  • Does not include cost of data collection in Flow Cytometry Core                                   
  • Isolation of MNCs is a separate charge

$128

Quantitation of CFU-ECs from human blood

  • Plating, culture and quantitation of CFU-ECs (in triplicate)
  • Isolation of MNCs is a separate charge

$240

Quantitation of ECFCs from human blood

  • Plating, maintanence, and quantitation of 3 wells for ECFCs
  • Isolation of MNCs is a separate charge

$141

Expansion and cryopreservation of ECFCs

  • Expansion of ECFC colonies to passage 2 and cryopreservation of cells

$510

 

Endothelial cell isolation from human umbilical veins

  • Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by poly-chromatic flow cytometry

$122

Brief endothelial phenotyping of endothelial cells by poly-chromatic flow cytometry analysis

  • Staining of cell line with endothelial antigen panel to confirm phenotype including controls
  • Does not include usage charge from Flow Cytometry Core

$280

Endothelial progenitor cell clonogenic assays

  • Limiting dilution analysis of up to 6 conditions in triplicate

$330

Endothelial progenitor cell clonogenic assays

  • Single cell analysis of up to 6 X 96 well plates
  • Does not include sorting charge from Flow Cytometry Core
  • Does not include cost of cell line

$240

Endothelial cell proliferation assays (96 well format)

  • Thymidine incorporation in 96 well plates for up to 10 conditions (including controls) with 6 replicates
  • Does not include cost of cell line

$515

Endothelial cell migration assays

  • Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate
  • Does not include cost of cell line

$315

Tube forming assays with human endothelial cells

  • Up to 10 wells assayed for endothelial cell tube formation

 

$265

Retroviral transduction of primary human endothelial cells with fluorescently labeled proteins

  • Retroviral transduction with eGFP or dsRed and expansion of cell line

$267

Cryopreserved human umbilical cord blood and peripheral blood derived ECFC cell line

$267

Cryopreserved HUVEC cell line

$740

 

Assessment of endothelial cell apoptosis via TUNEL                                                        


  • TUNEL assessment of endothelial cell apoptosis.                                                              
  • Up to 6 conditions in triplicate, including controls
  • Does not include cost of cell line.

$485

Mouse carotid artery ligation


  • Up to 6 conditions in triplicate, including controlsLigation of mouse left carotid artery in up to six mice. Post-surgical monitoring of animals, removal of stitches, harvest of the left & right common carotid artery tissue and fixation of the harvested carotid arteries with 10 percent formalin.
  • Does not include cost of mice or per diems, which will be calculated and charged on an individual basis.

$500

 

CD34+ Magnetic Cell Separation and Cryopreservation of CD34+ Cells ($500/1e6)

  • Magnetic cell separation of CD34+ cells from human bone marrow, peripheral blood or umbilical cord blood MNCs, including purity determination by flow cytometry and cryopreservation of CD34+ recovered cells.

$500

AC133+ Magnetic Cell Separation and Cryopreservation of AC133+ Cells
($500, exact cost to be determined per MNCs).

  • Magnetic cell separation of AC133+ cells from human bone marrow, peripheral blood or umbilical cord blood MNCs, including purity determination by flow cytometry and cryopreservation of AC133+ recovered cells.
Cost to be determined per study and antibodies used
Custom design phenotypic analysis staining panels for the identification of EPCs or CPCs in different tissues

  • Up to 8 colors available.

$70/hour

Protocol design and development

  • Project-specific consulting for investigators interested in the implementation of flow cytometry utilizing EPCs and circulating progenitor cells (CPCs) as biomarkers in pre-clinical and clinical phase applications.
$60/hour Detailed/retrospective flow cytometry analysis utilizing FloJo

  • Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CPC phenotyping for newly identified cell sub-populations of interest.
$30/hour Technician time

*Users outside the IU School of Medicine or IU Simon Cancer Center will be charged an additional 20%.