Angiogenesis, Endothelial & Pro-Angiogenic Cell CoreServices
The Angiogenesis, Endothelial & Pro-Angiogenic Cell Core conducts validated and highly reproducible in
vitro and in vivo angiogenesis assays which include:
In Vitro Assay Systems in Endothelial Cell and Progenitor Cell Biology
- Endothelial cell isolation from human umbilical veins
- Endothelial colony forming cell isolation from human umbilical cord blood and human peripheral blood
- Endothelial colony forming cell clonogenic assays
- Endothelial cell proliferation assays
- Endothelial cell migration assays
- Tube forming assays with human endothelial cells
- co-culture tube forming assays using transwell systems
- stromal cell/endothelial cells
- tumor cells/endothelial cells
- iInflammatory cells/endothelial cells
- Ex vivo aortic outgrowth model of capillary structures
- 5- & 7-color poly-chromatic flow cytometry acquisition and analysis of human umbilical cord blood and peripheral blood mononuclear cells (MNCs)
- Assessment of endothelial cell apoptosis via TUNEL

In Vivo Assay Systems in Endothelial Cell and Progenitor Cell Biology
- Transplantation of human endothelial colony forming cells into immunodeficient mice