Angiogenesis and Endothelial Progenitor CellServices

The Angiogenesis and Endothelial Progenitor Cell Core conducts validated and highly reproducible in vitro and in vivo angiogenesis assays which include:

In Vitro Assay Systems in Endothelial Cell Biology

  1. Endothelial cell isolation from human umbilical veins
  2. Endothelial colony forming cell isolation from human umbilical cord blood and human peripheral blood
  3. Endothelial colony forming cell clonogenic assays
  4. Endothelial cell proliferation assays
  5. Endothelial cell migration assays
  6. Tube forming assays with human endothelial cells
    • co-culture tube forming assays using transwell systems
    • stromal cell/endothelial cells
    • tumor cells/endothelial cells
    • iInflammatory cells/endothelial cells
  7. Ex vivo aortic outgrowth model of capillary structures
  8.  5- & 7-color poly-chromatic flow cytometry acquisition and analysis of human umbilical cord blood and peripheral blood mononuclear cells (MNCs)
  9.  Assessment of endothelial cell apoptosis via TUNEL

     

     

In Vivo Assay Systems in Endothelial Cell Biology

  1. Mouse matrigel plug model
  2. Bone marrow contribution to tumor microenvironments utilizing chimeric mice with green fluorescent protein-labeled bone marrow cells
  3. Transplantation of human endothelial colony forming cells into immunodeficient mice