Angiogenesis, Endothelial & Pro-Angiogenic Cell CoreServices

The AEPCC is a state-of-the-art facility that has been established through the IU Simon Cancer Center (NCI-designated) to conduct validated and highly reproducible in vitro and in vivo angiogenesis, hematopoietic and multi-parametric flow cytometry assays. These assays function as experimental platforms for understanding the basic mechanisms of hematopoiesis, angiogenesis and discovering compounds that inhibit new blood vessel formation in tumor microenvironments.

The specific duties of the AEPCC are:

  • Provide consultation and guidance for the design and analysis of experiments to define cell subsets that participate in angiogenesis (biomarkers for a variety of human diseases).
  • Prepare research and human clinical blood samples for acquisition in the Flow Cytometry Resource Facility and conduct detailed cell surface analysis using FlowJo software in AEPCC.
  • Perform in vitro assays for angiogenesis.
  • Perform in vitro clonal assays of hematopoietic progenitor cell function.
  • Instruct interested investigators in the methods of hematopoietic stem and progenitor cell analysis and in a variety of angiogenesis assays.
  • Develop new methods for detecting pro-angiogenic cells (but non-vessel forming) and angiogenic cells (vessel-forming) from patient blood and tissue samples.

Chargeback rates for services of the Angiogenesis, Endothelial & Pro-Angiogenic Cell Core, effective July 1, 2012, are below:

 

Charge* Services

$90

Ficoll preparation of human blood MNCs

  • Preparation includes underlaying of up to 100mls of blood, washes and cell count

$65

CPT preparation of human blood MNCs

  • Preparation includes processing of up to 48mls of blood in CPT tubes supplied by the user, washes and cell count

$210

7-color multi-parametric flow cytometry analysis of human blood MNCs

  • Staining of MNCs with 7 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
  • Does not include cost of data collection in Flow Cytometry Core
  • Isolation of MNCs is a separate charge
$170

5-color multi-parametric flow cytometry analysis of human blood MNCs                                                                          

  • Staining of MNCs with 5 antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.                                                                                                                                                                 
  • Does not include cost of data collection in Flow Cytometry Core                                   
  • Isolation of MNCs is a separate charge

$40

Quantitation of CFU-GEMM from human blood

  • Plating, culture and quantitation of CFU-GEMMs (in triplicate)
  • Isolation of MNCs is a separate charge

$255

Quantitation of ECFCs from human blood

  • Plating, maintanence, and quantitation of 3 wells for ECFCs
  • Isolation of MNCs is a separate charge

$160

Expansion and cryopreservation of ECFCs

  • Expansion of ECFC colonies to passage 2 and cryopreservation of cells

$510

 

Endothelial cell isolation from human umbilical veins

  • Isolation of endothelial cells from human umbilical veins, expansion to passage 1 and phenotypic confirmation by poly-chromatic flow cytometry

$125

Brief endothelial phenotyping of endothelial cells by poly-chromatic flow cytometry analysis

  • Staining of cell line with endothelial antigen panel to confirm phenotype including controls
  • Does not include usage charge from Flow Cytometry Core

$385

Endothelial cell proliferation assays (96 well format)

  • Thymidine incorporation in 96 well plates for up to 10 conditions (including controls) with 6 replicates
  • Does not include cost of cell line

$520

Endothelial cell migration assays

  • Haptotaxis in transwells for up to 8 conditions (including controls) in triplicate
  • Does not include cost of cell line

$375

Tube forming assays with human endothelial cells

  • Up to 10 conditions assayed for endothelial cell tube formation
  • cost of cell line not included in price of assay

$475

Cryopreserved human umbilical cord blood derived CD34+ cells

• Staining of MNCs with indirect CD34+ MACS kit, cryopreservation of isolated cells per 1X106 cells and analysis of the cells for purity using FlowJo

$225

Cryopreserved human umbilical cord blood and peripheral blood derived ECFC cell line - 1X106 cells/vial

$300

Cryopreserved HMVEC cell line - 1X106 cells/vial

$150

Cryopreserved HUVEC cell line - 1X106 cells/vial

$350

Cryopreserved PMVEC cell line - 1X106 cells/vial

$745

Assessment of endothelial cell apoptosis via TUNEL                                                        


  • TUNEL assessment of endothelial cell apoptosis                                                              
  • Up to 6 conditions in triplicate, including controls
  • Does not include cost of cell line

Cost to be determined per study and antibodies used
Custom design phenotypic analysis staining panels for the identification of EPCs or CHSPCs in different tissues

  • Up to 8 colors available.

$70/hour

Protocol design and development

  • Project-specific consulting for investigators interested in the implementation of multi-parametric flow cytometry utilizing EPCs and circulating hematopoietic stem and progenitor cells (CHSPCs) as biomarkers in pre-clinical and clinical phase applications.
$60/hour Detailed/retrospective flow cytometry analysis utilizing FloJo

  • Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell sub-populations of interest.
$30/hour Technician time
*Users outside the IU School of Medicine or IU Simon Cancer Center will be charged an additional 20 percent.