Bio-PlexServices
Applications
Premixed combinations of beads are available to measure:
human, mouse, and rat cytokines & chemokines from serum, plasma, and cell culture supernatants
(NOTE: serum is strongly preferred over plasma. If plasma must be collected, please use EDTA or citrate or as the anticoagulant. DO NOT use heparin.)
Other applications include multiplex assays for:
- specific phosphorylation states and expression
- nucleic acids
- SNP analysis
- pathogen detection
- strain typing
- haplotyping
- gene expression
If the specific combination of beads for one’s desired assay is not available, Bio-Rad offers beads coated with carboxyl groups (-COOH), which are ready to couple to the investigator’s antibody, antigen, receptor, ligand, enzyme or nucleic acid of choice.
Each kit contains one 96-well plate capable of analyzing 78 samples in singlet or 39 samples in duplicate. Standards and blanks (run in duplicate) occupy 18 wells of each 96-well plate.
Equipment
Bio-Plex 200 System with High Throughput Fluidics (HTF), including:
- Bio-Plex array reader
- Pro II Wash Station automatic plate washer, accommodating both magnetic or vacuum-based assays
- Bio-Plex Manager Standard software
- PC and monitor
The core provides:
- Communication between user and Bio-Rad regarding kit selection, if needed
- Assistance with experimental design, etc., through discussions with user and BioRad, if requested
- Storage of kits purchased by users, if desired
- Receipt and analysis of samples collected by users
- All reagents & buffers required to run the samples (excluding kits)
- Analysis of raw data and possible optimization of standard curves using vendor software
- Delivery of data to user in an excel spreadsheet
- Preparation of report including concentration of target(s) and standard curve fit