Indiana University Melvin and Bren Simon Cancer Center

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You are here: HOME > Researchers > Facilities > Immunohistochemistry > Services

ImmunohistochemistryServices

The IHC Core is available for consultations and review of microscopic lesions and immunohistochemical stain results upon scheduling a meeting. Stop by with your materials and slides, result tables and files, images, etc. Please schedule a specific time and contact Lee Ann Baldridge at 491-6324. To ensure timely completion of large projects (those involving analyses from >30 animals), please contact the core one month prior to sample submission to discuss the goals of pathologic analysis, research questions posed, and types of analyses

The core offers:

• IHC and IMF for formalin fixed tissues (developed protocols)
• Macrophages: Mac-1, Mac-2, CD68, HAM 56, F4/80, myeloperoxidase
• Apoptosis: Cleaved Caspase 3, PARP
• Proliferation: Ki67, PH3, Cyclin B1, and PCNA
• Vascular Markers: Factor VIII, vWF, CD31, CD34, CD105, VegF, iNOS
• Cell Signaling Pathway Markers: b catenin, GSKb3, and Stat3
• Proliferating cell detection with BRDU labeling
• Apoptotic cell detection with Activated Caspae 3
• Development of new IHC and IMF protocols for detection of antigens in frozen or formalin fixed tissues
• One trial run includes three different types of antigen retrieval (for formalin fixed tissues), positive and negative controls, and three different concentrations of antibody.
• The number of available IHC and IMF stains is continually increasing.  

Routine Immunohistochemistry Staining

Standard protocols have been developed for a number of antibodies (contact Lee Ann Baldridge at 491-6324 ). Sections of paraffin-embedded tissue to be stained should be brought to the Clarian Pathology IHC Core Laboratory, fourth floor, between 8 a.m. and 4:30 p.m. Monday through Friday. The tissue must be accompanied by a completed work order form. If tissue sections are not available, the paraffin block may be brought to the core laboratory for sectioning.

Protocol Development for Novel Antibodies

The laboratory assists in the development of protocols for novel antibodies. Investigators should meet with the core director and scientific advisors to discuss this service. The investigator should be prepared to provide the antibody, references of its use in immunohistochemistry, positive and negative controls, information from Western analyses, and sections of the tissues to be stained, if available.