The Angio BioCore (ABC) is a state-of-the-art facility that has been established through the IU Simon Comprehensive Cancer Center to conduct validated and highly reproducible in vitro and in vivo angiogenesis, endothelial, hematopoietic and multi-parametric flow cytometry assays and their role in normal and patient-related hematologic and cardiovascular disorders.
In addition to possessing the in vitro and in vivo assays that define the endothelial progenitor outgrowth cells that possess in vivo vessel forming ability, the ABC recently stringently defined a population of pro-angiogenic and anti-angiogenic circulating hematopoietic stem and progenitor cells (CHSPCs) that has been shown to regulate angiogenesis. Discovery of these novel CHSPC subsets demonstrates the uniqueness and strength of the approach by the ABC that requires both phenotypic and functional data to validate specific circulating cells that participate in new blood vessel formation.
The specific assays offered by the ABC function as quantitative analytical tools, potential biomarkers of several hematopoietic diseases, and as experimental platforms for understanding the basic mechanisms of angiogenesis and the interplay between the endothelial and hematopoietic systems. For example, not only are CHSPCs critical for normal and abnormal angiogenesis, but we and others have reported that certain endothelial cells are critical for CHSPC expansion ex vivo and that endothelial cells promote CHSPC engraftment post-ablation.
The ABC houses the IncuCyte ZOOM and S3, from Sartorius, that allows the automated, extended, real-time assessment of cellular behavior under various experimental conditions. It has multiple capabilities of real time quantitative analysis for multiple biological processes via several different assays such as angiogenesis, apoptosis, cell migration/invasion, immune function, and 3D spheroid formation. The ABC also houses the Seahorse XFp analyzer, from Agilent Technologies, which allows users to perform routine tests of metabolic pathways in real time that measure oxygen consumption rate (OCR), an indicator of mitochondrial respiration, as well as extracellular acidification rate (ECAR), which is largely the result of glycolysis.
The ABC serves to directly perform all of the assays required to analyze research samples, and as a consultation, education, and new assay development site for scientists within and outside the IU School of Medicine and Indiana University.
Furthermore, the ABC is one of only five nationally recognized National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Cooperative Centers of Excellence in Hematology and is a core of the Indiana Clinical and Translational Sciences Institute (CTSI).