Clinical Pharmacology Analytical Core

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The mission of the Clinical Pharmacology Analytical Core (CPAC) is to facilitate the research and development of drugs and new chemical entities (NCEs) in support of internal and external investigators, in particular, IU School of Medicine, IU Simon Comprehensive Cancer Center (IUSCC), and Purdue University Center for Cancer Research members.

Description: The Clinical Pharmacology Analytical Core’s (CPAC) primary service is to develop methods to quantify drugs, metabolites, and new chemical entities (NCEs) from a variety of biological matrices by utilizing state-of-the-art ultra-performance liquid chromatography tandem mass spectrometer (UPLC-MS/MS). CPAC is aimed to assist investigators by providing detailed information on in vivo pharmacokinetics (PK), drug interactions, in vitro drug metabolic stability, formulation optimization, protein binding, and drug purity verification.

The drug discovery process should provide a delicate balance between the chemistry, pharmacology, and PK of the drug/NCE. Relevant PK and metabolism studies should be conducted in small animal models or in vitro systems before first drug administration in humans. This allows for the iterative process of implementing structural changes to the drug molecule to optimize the activity of the drug and its pharmacological and PK properties prior to moving to the more regulated and expensive clinical phase of drug development. PK plays an important role in the determination of drug action. PK and metabolism data provide important information to guide drug design and treatment in pre-clinical drug discovery (bench) as well as in clinical drug development and treatment (bedside) with efforts focused on evaluation of toxicity and efficacy of new drug candidates. CPAC’s technology and expertise supports investigators research and development of safe and more efficacious drug treatment.

Instruments: CPAC houses state-of-the-art instrumentation for quantification of drugs, metabolites, and NCEs as an invaluable resource for investigators in the cancer center. The core has three (U) HPLC-MS/MS instruments: Sciex 6500+ QTRAP, Sciex 5500 QTRAP, and Thermo TSQ Quantum Ultra.

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