Flow Cytometry

Services

Since the inception of this facility, there have been many requests for new services and new techniques have been added. The personnel and instrumentation available at the facility are capable of performing virtually any flow cytometric assay requested by IU Simon Comprehensive Cancer Center investigators.

Available instrumentation includes:

Multispectral Imaging Flow Cytometer:

  • Amnis Image StreamX MKII

Analyzers:

  1. BD LSRFortessa (X-20)
    • 3 lasers (407, 488, 630)
    • 2 light scatter parameters
    • 11 colors
  2. BD LSRFortessa (Fortessa)
    • 4 lasers (407, 488, 561, 630)
    • 2 light scatter parameters
    • 16 colors
Spectral Analyzer:
  1. Cytek Aurora
    • 4 lasers (407, 488, 561, 630)
    • Spectral cytometer
    • 3 light scatter parameters (SSC-Violet, SSC-Blue,FSC-Blue)
    • 48 parameters
    • Plate reader

Sorters:

  1. BD FACSAria
    • 3 lasers (407, 488, 630nm)
    • 2 light scatter parameters
    • 10 colors
  2. BD SORP Aria
    • 5 lasers (355, 405, 488, 552, and 637nm)
    • 2 light scatter parameters
    • 17 parameters
  3. BD FACSAria Fusion
    • 4 lasers (405, 488, 561, 640nm)
    • 2 light scatter parameters
    • 18 parameters

Available services include:

  1. Multiparameter (up to 16 colors) immunofluorescence analysis and cell sorting
  2. DNA content and cell cycle analysis
  3. Simultaneous immunofluorescence and cell cycle analysis
  4. Single cell sorting
  5. Cell sorting (bulk or single cells) based on position of cells in cell cycle
  6. Apoptosis analysis (Annexin V/PI, Caspase, TUNEL and other assays)
  7. BrdU incorporation
  8. Viability assays
  9. Chromosome analysis (univariate and bivariate)
  10. Receptor - ligand interactions
  11. Kinetic analyses
  12. Off-line data analysis

To cite Flow Cytometry instrumentation in papers and grants, you may include the following addresses:

BD Biosciences
2350 Qume Drive
San Jose, CA 95131-1807

Amnis Corp. (part of EMD Millipore)
645 Elliott Ave. W., Suite 100
Seattle, WA 98119

Cytek Biosciences
47215 Lakeview Boulevard
Fremont, CA 94538-6407