IU Genome Editing Center

Policies & Procedures

IUGEC service charges are as follows (FY 2021).

Model generation services

Category

Service

Units

Internal

External Non-Profit

External Industry

Mouse Model

CRISPR-mediated insertion/deletion (indel)

Per Project

 $7,000

 $8,400

 $9,660

Mouse Model

Missense or nonsense mutation

Per Project

 $12,000

 $14,400

 $16,560

Mouse Model

Insertion of small DNA elements (< 90 nucleotides)

Per Project

 $12,000

 $14,400

 $16,560

Mouse Model

CRISPR-mediated large deletion

Per Project

 $14,500

 $17,400

 $20,010

Mouse Model

Conditional allele

Per Project

 $24,000

 $28,800

 $33,120

Mouse Model

Insertion of large DNA elements (< 800 nucleotides)

Per Project

 $19,500

 $23,400

 $26,910

Mouse Model

Insertion of large DNA elements (> 800 nucleotides)

Per Project

 $26,000

 $31,200

 $35,880

Mouse Model

Insertion of large DNA elements at the ROSA26 locus

Per Project

 $20,500

 $24,600

 $28,290

Transformed Cell Line

CRISPR-mediated insertion/deletion (indel)

Per Project

 $7,500

 $9,000

 $10,350

Transformed Cell Line

Missense or nonsense mutation

Per Project

 $7,750

 $9,300

 $10,695

Transformed Cell Line

Insertion of small DNA elements (< 90 nucleotides)

Per Project

 $8,000

 $9,600

 $11,040

Transformed Cell Line

CRISPR-mediated large deletion

Per Project

 $8,500

 $10,200

 $11,730

Transformed Cell Line

Conditional allele

Per Project

 $17,000

 $20,400

 $23,460

Transformed Cell Line

Insertion of large DNA elements (< 800 nucleotides)

Per Project

 $18,000

 $21,600

 $24,840

Transformed Cell Line

Insertion of large DNA elements (> 800 nucleotides)

Per Project

 $19,000

 $22,8000

 $26,220

Transformed Cell Line

Insertion of large DNA elements at the ROSA26 locus

Per Project

 $18,000

 $21,600

 $24,840

Other

Statement-of-work fee

Per Service

 $2,000

 $2,400

 $2,760

 

 

 

 

 

 

A la carte services

Category

Service

Units

Internal

External Non-Profit

External Industry

Transgenic Services

Pronuclear injection into C57BL/6 embryos

Per Service

 $5,986

 $7,183.20

 $8,260.68

Transgenic Services

Sperm cryopreservation (with verification)

Per Service

 $1,907

 $2,288.40

 $2,631.66

Transgenic Services

Sperm cryopreservation (without verification)

Per Service

 $707

 $848.40

 $975.66

Transgenic Services

Cryopreservation of IVF embryos

Per Service

 $805

 $966

 $1,110.90

Transgenic Services

In vitro fertilization and embryo transfer

Per Service

 $1,890

 $2,268

 $2,608.20

Transgenic Services

Embryo thaw and transfer

Per Service

 $964

 $1,156.80

 $1,330.32

Transgenic Services

Cryo storage

Per Service

 $77

 $92.40

 $106.26

Other

Design

Per Hour

 $100

 $120

 $138

Technology development

Category

Service

Units

Internal

External Non-Profit

External Industry

Other

Technology development

Per service

cost

N/A

N/A

 
Procedures

For the generation of genetically modified mouse models and cell lines, fill out the IUGEC Project Information form and the IUGEC Model Registration form. Send the IUGEC Project Information form back to spellet@iu.edu and the IUGEC Model Registration form to spelleti@iu.edu and somiacuc@iupui.edu (IUPUI) or biacuc@indiana.edu (Bloomington). Make sure the mouse model or cell line you want the IUGEC to engineer is listed on your IBC (IBC@iu.edu) and IACUC protocols.

Upon receipt of the IUGEC Project Information form, the IUGEC will design a targeting strategy and produce a Statement of Work (SOW). The SOW contains information detailing the targeting strategy, the genotyping strategy, and others. A SOW fee will then be charged to the investigator. This fee serves as deposit and will be deducted in full upon completion of the model. This fee will not be reimbursed should the investigator decide not to pursue the project with the IUGEC or use our a la carte services for the generation of the model.

For a la carte services, fill out the IUGEC Transgenic Services form and send the information back to hanchen@iupui.edu. If you are using our pronuclear injection service, please fill out IUGEC Model Registration form and send the form to spelleti@iu.edu and somiacuc@iupui.edu (IUPUI) or biacuc@indiana.edu (Bloomington). Make sure the mouse model engineered is listed on your IBC and IACUC protocols. 

  1. Model generation services
  • Mouse models

 For the generation of mouse models with user-defined mutation, the IUGEC will: 

  • Design a targeting strategy
  • Generate reagents used to create the mouse model
  • Test genotyping strategy
  • Generate of founder mice
  • Genotype founder mice
  • Analyze founders for off-target cleavage (when using CRISPR technology for the generation of the model)
  • Test germline transmission of the genetic modification
  • Cryopreserve the mouse model (sperm)
  • Deliver the mouse model and documentation related to the model to investigator

The IUGEC guarantees the generation of the mouse models unless the mutation is not compatible with embryonic development or is lethal early in the life of the animal. IUGEC reserves the right not to take on projects with low chance of success. The IUGEC provides C57BL/6 mice. If an investigator needs other strains, he or she will need to pay for them. 

  • Cell line models

For the generation of cell models with user-defined mutation, the IUGEC will: 

  • Design a targeting strategy
  • Generate reagents used to create the cell model
  • Test genotyping strategy
  • Transduced cells and isolate clones
  • Genotype clones
  • Cryopreserve clones
  • Deliver the cell model and documentation related to the model to investigator

The IUGEC guarantees the generation of the cell line model unless the cells cannot be single cell cloned (which must be tested by the investigators prior to engineering the model), or the mutation is lethal or prevents the growth of the cells. The IUGEC reserves the right not to take on projects with low chance of success. 

  1. A la carte services
  • Pronuclear injection

For pronuclear injection, investigators provide the DNA construct to the IUGEC. The IUGEC will: 

  • Gel purify traditional transgene constructs
  • Harvest single cell embryos
  • Microinject the embryos with the transgene or CRIPSR construct
  • Transplant the microinjected embryos into pseudo-pregnant females
  • Perform Cesarean sections as required
  • Provide the resulting pups at weaning age to the investigator for analyses

The IUGEC guarantees 10 births per round of pronuclear injection when using C57BL/6. embryos (historic average is 14 births per round). IUGEC reserves the right not to take on projects with low chance of success. The IUGEC provides C57BL/6 mice. If an investigator needs other strains, he or she will need to pay for them. 

  • Sperm cryopreservation with verification

For sperm cryopreservation with verification, investigators provide a minimum of two male mice. The IUGEC will: 

  • Harvest sperm from the donor
  • Produce 40 straws of frozen sperm
  • Verify successful freezing via in vitro fertilization using the thawed sperm and donor eggs from the relevant strain of mice; verification entails development of 2-cell embryos following in vitro fertilization

The verification is the guarantee for this service. 

  • Sperm cryopreservation without verification

For sperm cryopreservation without verification, investigators provide a minimum of two male mice. The IUGEC will: 

  • Harvests sperm from the donor
  • Produce 40 straws of frozen sperm

The core guarantees mobile sperm upon test thawing. Cryopreservation activities are based on a good faith agreement between the investigators and the IUGEC. Thus far, the core has not encountered any difficulties in the recovery of cryopreserved samples which it has generated. However, we cannot guarantee that all samples can be recovered following long-term storage. 

  • In vitro fertilization and embryo transfer

For in vitro fertilization and embryo transfer, investigators provide either frozen sperm samples or a minimum of two living mice. The IUGEC will: 

  • Thaw the frozen sperm or harvests fresh sperm from the donor Performs in vitro fertilization
  • Cultures embryos to 2-cell stage
  • Transplant the 2-cell embryos into pseudo-pregnant females Performs Cesarean sections as required
  • Provide the resulting pups at weaning age to the investigator for analyses

If no births are obtained, a second round (optimized based on the results from the first round) will be performed for no additional charge. Subsequent efforts will require additional billing. 

  • Embryo thaw and transfer

For embryo thaw and transfer, investigators provide the frozen embryos. The IUGEC will: 

  • Transplant the 2-cell embryos into pseudo-pregnant females
  • Perform Cesarean sections as required
  • Provide the resulting pups at weaning age to the investigator for analyses

If no births are obtained, a second round (optimized based on the results from the first round) will be performed for no additional charge. Subsequent efforts will require additional billing. 

  • Cryo storage

Cryopreservation activities are based on a good faith agreement between the investigators and the IUGEC. Thus far, the core has not encountered any difficulties in the recovery of cryopreserved samples which it has generated. However, we cannot guarantee that all samples can be recovered following long-term storage. 

Policies 

  • Confidentiality

All data, results and models generated by IUGEC will be accessible only to the investigator(s) involved in the project. Data will not be shared in any way without permission from the investigator(s). 

  • Cost Recovery/Payment

All users are charged a fee for services provided by the IUGEC. All users of the IUGEC are expected to pay for the services provided. An IU account number or a purchase order number (PO#) must be provided at the start of the service. For a la carte services (conventional transgenic services), full payment is expected, regardless of the outcome of the service. For animal models or cell line models, full payment is expected if the model is generated if the mutation(s) engineered are not lethal to cells or deleterious to mouse development. If the mutations are lethal to cells or not compatible with mouse development, 75 percent of the cost will be covered by the user and 25 percent by the IUGEC. The IUGEC also reserves the right to decline taking on projects with low chance of success.  

  • Prioritization of work

All services are on a first-come first-served basis. Some projects may take longer to complete than others. The IUGEC reserves the right to modify schedule to minimize the number of mice used. 

  • Acknowledgement

The IUGEC should be acknowledged in all publications or grants describing the models generated by the center, and a copy of the publication should be provided to the center. The following statement should be added to any publications that used the IUGEC services: “This work was supported by the Indiana University Genome Editing Center at Indiana University School of Medicine."

  • Authorship

Authorship will be granted to individual(s) involved in the design and oversight of genome editing projects. Fees paid for services provided by the IUGEC do not preclude or replace authorship. 

  • Conflict resolution

The director of the IUGEC will discuss all issues with investigators and will seek to resolve them. In the event issues arise that cannot be resolved by the IUGEC director, the IUGEC Advisory Committee will resolve the issues. 

  • Submitting Material in Compliance with Regulatory Requirements

Material or health-related information submitted to IUGEC must be obtained in compliance with current regulatory and legal requirements, that users comply with all safety standards (IRB, IBC, IACUC, others) and fully disclose the potential hazards of materials submitted.