Preclinical Modeling and Therapeutics Core

Services

Celluar Response Technologies (CRT)

  • Provide consultation and guidance for the design and analysis of experiments to define biomarkers for a variety of human diseases
  • Prepare research and clinical blood and tissue samples (animal and human) for acquisition in the Flow Cytometry Resource Facility and conduct detailed cell surface analysis using FlowJo software
  • Perform in vitro functional assays (angiogenic tube-forming, proliferation, migration, invasion, etc) to study cellular function in multiple cell types
  • Instruct investigators in the methods of hematopoietic stem and progenitor cell analysis
  • Consult and train users on the acquisition and analysis of data using two IncuCyte Live-Imaging systems (ZOOM and S3)  and the Seahorse XFp

In Vivo Therapeutics (IVT)

Pharmacological Investigations:

In vitro toxicity testing

  • Utilize primary human hematopoietic cells and clonogenic progenitor assays to test effects of new compounds on normal cellular function

In vivo dose range finding

  • Coordinate pharmacokinetic studies with the Clinical Pharmacology Analytical Core (CPAC) in the School of Medicine
  • Evaluate efficacy and safety of therapies of new compounds and/or combination therapies to develop rational dosing regimes
  • Coordinate end-point analysis (necropsy and histology) with Preclinical Histology Core in the IU School of Medicine

In vivo tumor growth kinetics

  • Facilitate development of novel humanized and  immunocompetent xenograft models
  • Document tumor progression of previously untested tumors prior to drug efficacy and safety studies in immunodeficient mice.
  • Utilize the IVIS SpectrumCT optical imaging system to document tumor growth in orthotopic models via bioluminescence and fluorescence modalities. 

In vivo drug efficacy/tumor xenograft model

  • Establish tumors and administer drug regimen
  • Document tumor progression in vehicle- and drug-treated mice
  • Coordinate longitudinal imaging with the In vivo imaging core 
  • Coordinate end-point analysis (necropsy and histology) with Preclinical Histology Core in the IU School of Medicine

Cellular Therapies:

  • Radiation of mice prior to transplantation
  • Intravenous injection of hematopoietic stem cells into mice
  • Provide investigator blood and tissue samples from transplanted mice
  • Coordinate analysis of multi-lineage engraftment in transplanted mice via flow cytometry with the CRT team.
FEES

 

Cell Response Technologies

Cell Separation
Services

Internal IU Faculty rates

External rates
Ficoll Preparation of Human Blood MNCs (20mls)
  • Preparation includes underlaying of up to 20mls of blood, washes and cell count
$60 $90
Ficoll Preparation of Human Blood MNCs (100mls)
  • Preparation includes underlaying of up to 100mls of blood, washes and cell count
$110 $165
CPT Preparation of Human Blood MNCs
  • Preparation includes processing of up to 48mls of blood in CPT tubes (supplied by the user), washes and cell count
$70 $10
Plasma Separation and Freeze Down
  • Preparation includes centrifuging and freeze down of up to eight vials of plasma
$20 $30
MNC Separation and Freeze Down (with 7C Flow)
  • Preparation includes freeze down of up to five vials of MNCs
$20 $30
MNC Separation and Freeze Down (without 7C Flow)
  • Preparation includes isolation of MNCs and freeze down of up to five vials of MNCs
$70 $105
Multi-Parametric Flow Cytometry
Services Internal IU Faculty rates External rates
7-Color MPFC Analysis of Human Blood MNCs
  • Staining of MNCs with seven antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo
  • Does not include cost of data collection in Flow Cytometry Core
  • Isolation of MNCs is a separate charge
$200 $300
Up to 8-Color MPFC Analysis of Human Blood MNCs, Bone Marrow MNCs or Cell Lines

Can design any panel for the specific PI's interests

  • Staining of MNCs with eight antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
  • Does not include usage charge from Flow Cytometry Facility
  • Isolation of cells is a separate charge
TBD TBD
Cell Culture
Services Internal IU Faculty rates External rates
Quantitation of CFU-GEMMs from Human Blood
  • Plating, culture and quantitation of CFU-GEMMs (in triplicate)
  • Isolation of MNCs is a separate charge
  • Blood provided by Investigator
$45 $67.50
Quantitation of Endothelial Outgrowth Cells (EOCs) from Human Blood
  • Plating, maintanence and quantitation of three wells for EOCs
  • Isolation of MNCs is a separate charge
  • Blood provided by Investigator
$285 $427.50
Expansion and Cryopreservation of Endothelial Outgrowth Cells (EOCs)
  • Expansion of EOC colonies to passage 2 and cryopreservation of cells
  • Blood provided by Investigator
$215 $322.50
Functional Assays
Services Internal IU Faculty rates External rates
Tube Forming Assays (Matrigel) with Human Endothelial Cells
  • Up to 10 conditions in triplicate assayed for endothelial cell tube formation
  • Does not include cost of cell line
$225 $337.50

Proliferation, Migration, Invation Assay on various cells
Culture, and plating of cells fro various assays
*Investigator must provide cell line

TBD TBD
Incucyte S3
Services Internal IU Faculty rates External rates
Charge per 1 plate/day $44 $55
Metabolism Studies/Seahorse XFp
Services Internal IU Faculty rates External rates
Cell Characterization, FCCP Titration $175 $262.50
Cell Glycolysis Assay-Stress Test $190 $285
Mitochondrial Respiration-Mito Stress Test $175 $262.50
Cell Energy Phenotype Assay $155 $232.50
Mito Flex Fuel Assay $180 $270
Training for Equipment Use $255 $382.50
*The price of the assays include the cost of the Seahorse consumables and kits without any overhead charge.

Miscellaneous
Services Internal IU Faculty rates External rates
Technician time $40/hour  
Equipment Fee $25  
Retrospective analysis of FCS3.0 files
  • Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell
    sub-populations of interest
$60/hour  

 

InVivo Therapeutics

The core is funded, in part, by grants and contracts of the investigators who utilize the facility. All IUSCCC members receive discounted rates. Due to the variety of in vivo models and drug regimens requested, the specific costs of a study will be calculated once the study design is finalized.

Weekend/after-hours/holiday rates will be two times the regular procedure rate, with minimum charge per trip ($191)

External rates TBD upon consultation.
Service Internal IU faculty rates
Tissue culture fees $43.51/hour
Lentiviral transduction of cell line (includes cell transduction and flow cytometry verification) $43.51/hour + flow lab fees
Methylene blue assay for cell viability after drug treatment $43.51/hour
Tissue culture supply fees (tubes, pipettes, tips, trypsin, etc.) $6.22/hour spent w/TC
Mycoplasma testing cell line (required for invivo studies) $74.87/test
Cell line banking, authentication, distribution Charges dependent on effort and supplies
Experimental technique training $43.51/hour
Cost analysis and billing $43.51/hour
Draft and submit study amendments $43.51/hour
Drug formulation (15 min increments) $43.51/hour
Randomization & rehousing of animals $43.51/hour
Study tasks with staggered times (dosing, bleeding, etc) $43.51/hour
Surgical procedures/orthotic implant $56.50/hour/person
NSG mice $30.66/mouse
NSGS mice $38.20/mouse
NRG mice $38.58/mouse
Boy J mice $36.04/mouse
C57 / Boy J F1 $34.27/mouse
C57BL / 6J mice $29.67/mouse
Biohazard mouse room per diems $1.30/cage/day
Conventional mouse room per diems $0.98/cage/day
Data entry, analysis and graphing $87.02/week of study
Irradiation prior to experiment $43.51/hour
Irradiation of cells $43.51/hour
Irradiation “late” fee if >15 minutes late (with no written notice in advance) $43.51/hour + additional 10% for each subsequent occurrence
Tattoo fees (animal identification) $2.48/mouse
Inhalation anesthesia fees (per hour to cover O2, isoflurane) $11.42/hour
Manual Palpation (for tumors) $1.35/mouse
Caliper tumors $4.10/mouse
IV injection $6.84/mouse
SQ injection $4.10/mouse
IP, ID, or IM injection $2.74/mouse
PO dose $2.74/mouse
Body weight $1.35/mouse
Survival or terminal bleed $6.84/mouse
Euthanize and dispose (only) $1.35/mouse
Euthanize + gross necropsy $13.68/mouse
Euthanize + tumor (fixation) $13.68/mouse
Euthanize + full necropsy $21.05/mouse
Full necropsy + tissues in cassettes

$25.43/mouse

Cellularity and progenitor analysis (BM or spleen) $64.25/mouse **
Hematopoietic progenitor analysis (human UCB) $42.43/drug treatment **
IVIS Spectrum CT Rates:
IVIS Imaging $50.00/hr
IVIS Data Analysis $50.00/hr
IVIS Training $50.00/hr
** This is for estimation purposes only. Acutal billed cost will include all needed supplies + actual effort
Weekend/after-hours/holiday rates will be two times the regular procedure rate, with minimum charge per trip ($191)

If you are a cancer center member, please contact Kristina Taylor at kristtay@iu.edu or 317-274-3893, for additional rate information.