Preclinical Modeling and Therapeutics Core
Services
Celluar Response Technologies (CRT)
- Provide consultation and guidance for the design and analysis of experiments to define biomarkers for a variety of human diseases
- Prepare research and clinical blood and tissue samples (animal and human) for acquisition in the Flow Cytometry Resource Facility and conduct detailed cell surface analysis using FlowJo software
- Perform in vitro functional assays (angiogenic tube-forming, proliferation, migration, invasion, etc) to study cellular function in multiple cell types
- Instruct investigators in the methods of hematopoietic stem and progenitor cell analysis
- Consult and train users on the acquisition and analysis of data using two IncuCyte Live-Imaging systems (ZOOM and S3) and the Seahorse XFp
In Vivo Therapeutics (IVT)
Pharmacological Investigations:
In vitro toxicity testing
- Utilize primary human hematopoietic cells and clonogenic progenitor assays to test effects of new compounds on normal cellular function
In vivo dose range finding
- Coordinate pharmacokinetic studies with the Clinical Pharmacology Analytical Core (CPAC) in the School of Medicine
- Evaluate efficacy and safety of therapies of new compounds and/or combination therapies to develop rational dosing regimes
- Coordinate end-point analysis (necropsy and histology) with Preclinical Histology Core in the IU School of Medicine
In vivo tumor growth kinetics
- Facilitate development of novel humanized and immunocompetent xenograft models
- Document tumor progression of previously untested tumors prior to drug efficacy and safety studies in immunodeficient mice.
- Utilize the IVIS SpectrumCT optical imaging system to document tumor growth in orthotopic models via bioluminescence and fluorescence modalities.
In vivo drug efficacy/tumor xenograft model
- Establish tumors and administer drug regimen
- Document tumor progression in vehicle- and drug-treated mice
- Coordinate longitudinal imaging with the In vivo imaging core
- Coordinate end-point analysis (necropsy and histology) with Preclinical Histology Core in the IU School of Medicine
Cellular Therapies:
- Radiation of mice prior to transplantation
- Intravenous injection of hematopoietic stem cells into mice
- Provide investigator blood and tissue samples from transplanted mice
- Coordinate analysis of multi-lineage engraftment in transplanted mice via flow cytometry with the CRT team.
FEES
Cell Response Technologies
Cell Separation
| Services |
Internal IU Faculty rates |
External rates |
|---|---|---|
Ficoll Preparation of Human Blood MNCs (20mls)
|
$60 | $90 |
Ficoll Preparation of Human Blood MNCs (100mls)
|
$110 | $165 |
CPT Preparation of Human Blood MNCs
|
$70 | $10 |
Plasma Separation and Freeze Down
|
$20 | $30 |
MNC Separation and Freeze Down (with 7C Flow)
|
$20 | $30 |
MNC Separation and Freeze Down (without 7C Flow)
|
$70 | $105 |
Multi-Parametric Flow Cytometry
| Services | Internal IU Faculty rates | External rates |
|---|---|---|
7-Color MPFC Analysis of Human Blood MNCs
|
$200 | $300 |
| Up to 8-Color MPFC Analysis of Human Blood MNCs, Bone Marrow MNCs or Cell Lines Can design any panel for the specific PI's interests
|
TBD | TBD |
Cell Culture
| Services | Internal IU Faculty rates | External rates |
|---|---|---|
Quantitation of CFU-GEMMs from Human Blood
|
$45 | $67.50 |
Quantitation of Endothelial Outgrowth Cells (EOCs) from Human Blood
|
$285 | $427.50 |
Expansion and Cryopreservation of Endothelial Outgrowth Cells (EOCs)
|
$215 | $322.50 |
Functional Assays
| Services | Internal IU Faculty rates | External rates |
|---|---|---|
Tube Forming Assays (Matrigel) with Human Endothelial Cells
|
$225 | $337.50 |
|
Proliferation, Migration, Invation Assay on various cells |
TBD | TBD |
Incucyte S3
| Services | Internal IU Faculty rates | External rates |
|---|---|---|
| Charge per 1 plate/day | $44 | $55 |
Metabolism Studies/Seahorse XFp
| Services | Internal IU Faculty rates | External rates |
|---|---|---|
| Cell Characterization, FCCP Titration | $175 | $262.50 |
| Cell Glycolysis Assay-Stress Test | $190 | $285 |
| Mitochondrial Respiration-Mito Stress Test | $175 | $262.50 |
| Cell Energy Phenotype Assay | $155 | $232.50 |
| Mito Flex Fuel Assay | $180 | $270 |
| Training for Equipment Use | $255 | $382.50 |
*The price of the assays include the cost of the Seahorse consumables and kits without any overhead charge.
Miscellaneous
| Services | Internal IU Faculty rates | External rates |
|---|---|---|
| Technician time | $40/hour | |
| Equipment Fee | $25 | |
Retrospective analysis of FCS3.0 files
|
$60/hour |
InVivo Therapeutics
The core is funded, in part, by grants and contracts of the investigators who utilize the facility. All IUSCCC members receive discounted rates. Due to the variety of in vivo models and drug regimens requested, the specific costs of a study will be calculated once the study design is finalized.
Weekend/after-hours/holiday rates will be two times the regular procedure rate, with minimum charge per trip ($191)
External rates TBD upon consultation.| Service | Internal IU faculty rates |
|---|---|
| Tissue culture fees | $43.51/hour |
| Lentiviral transduction of cell line (includes cell transduction and flow cytometry verification) | $43.51/hour + flow lab fees |
| Methylene blue assay for cell viability after drug treatment | $43.51/hour |
| Tissue culture supply fees (tubes, pipettes, tips, trypsin, etc.) | $6.22/hour spent w/TC |
| Mycoplasma testing cell line (required for invivo studies) | $74.87/test |
| Cell line banking, authentication, distribution | Charges dependent on effort and supplies |
| Experimental technique training | $43.51/hour |
| Cost analysis and billing | $43.51/hour |
| Draft and submit study amendments | $43.51/hour |
| Drug formulation (15 min increments) | $43.51/hour |
| Randomization & rehousing of animals | $43.51/hour |
| Study tasks with staggered times (dosing, bleeding, etc) | $43.51/hour |
| Surgical procedures/orthotic implant | $56.50/hour/person |
| NSG mice | $30.66/mouse |
| NSGS mice | $38.20/mouse |
| NRG mice | $38.58/mouse |
| Boy J mice | $36.04/mouse |
| C57 / Boy J F1 | $34.27/mouse |
| C57BL / 6J mice | $29.67/mouse |
| Biohazard mouse room per diems | $1.30/cage/day |
| Conventional mouse room per diems | $0.98/cage/day |
| Data entry, analysis and graphing | $87.02/week of study |
| Irradiation prior to experiment | $43.51/hour |
| Irradiation of cells | $43.51/hour |
| Irradiation “late” fee if >15 minutes late (with no written notice in advance) | $43.51/hour + additional 10% for each subsequent occurrence |
| Tattoo fees (animal identification) | $2.48/mouse |
| Inhalation anesthesia fees (per hour to cover O2, isoflurane) | $11.42/hour |
| Manual Palpation (for tumors) | $1.35/mouse |
| Caliper tumors | $4.10/mouse |
| IV injection | $6.84/mouse |
| SQ injection | $4.10/mouse |
| IP, ID, or IM injection | $2.74/mouse |
| PO dose | $2.74/mouse |
| Body weight | $1.35/mouse |
| Survival or terminal bleed | $6.84/mouse |
| Euthanize and dispose (only) | $1.35/mouse |
| Euthanize + gross necropsy | $13.68/mouse |
| Euthanize + tumor (fixation) | $13.68/mouse |
| Euthanize + full necropsy | $21.05/mouse |
| Full necropsy + tissues in cassettes |
$25.43/mouse |
| Cellularity and progenitor analysis (BM or spleen) | $64.25/mouse ** |
| Hematopoietic progenitor analysis (human UCB) | $42.43/drug treatment ** |
| IVIS Spectrum CT Rates: | |
| IVIS Imaging | $50.00/hr |
| IVIS Data Analysis | $50.00/hr |
| IVIS Training | $50.00/hr |
| ** This is for estimation purposes only. Acutal billed cost will include all needed supplies + actual effort | |
| Weekend/after-hours/holiday rates will be two times the regular procedure rate, with minimum charge per trip ($191) | |
If you are a cancer center member, please contact Kristina Taylor at kristtay@iu.edu or 317-274-3893, for additional rate information.
