Fabiana Perna, M.D., Ph.D.
975 W. Walnut St.
Indianapolis, IN 46202
Research Program Membership
Department of Medicine
Division of Hematology/Oncology
IU School of Medicine
Dr. Perna's research interests include:
The goal of the Perna lab is to develop precision CAR therapy by profiling the leukemia surface proteome and investigating how key epigenetic mutations shape a targetable cell surfaceome. By mining composite high-throughput expression data in genetic models and primary AML patient samples with a pipeline that she established for CAR target discovery, targets identified will be integrated into a personalized therapeutic platform, taking into account patient genetic mutational background. Her lab generated human genetic models of AML by retrovirally expressing key epigenetic mutations in CD34+ hematopoietic stem/progenitor cells and will utilize the IU School of Medicine HemOnc BioBank, a rich resource of banked and sequenced patient samples. Large scale multiomic studies, including biotin-labeling and glycoprotein-capturing enriching methods for surface proteins followed by label-free and multiplexed targeted MS analysis, single-cell RNA-seq analysis and human xenograft in immune-deficient mice in combination with flow cytometry and IHC validation will aid in unbiased identification of genetically-defined cell surface targets. Preliminary data unveiled aberrant expression of certain immune inhibitory receptors on leukemia cells that may suppress T cell activity. By knocking-down their expression, her lab will define their role in normal and malignant hematopoiesis and regulatory pathways under hypoxic conditions and engraftment as xenografts.
Ph.D. - Federico II University, Naples, Italy/Memorial Sloan Kettering Cancer Center, New York, NY 2010-2013
Post-doctoral Fellowship - Memoprial Sloan Kettering Cancer Center, New York, NY 2008-2013
M.D. - Federico II University, Naples, Italy 2004